Subject Details
Dept     : FTECH
Sem      : 3
Regul    : 2019
Faculty : Dr K Radhika
phone  : NIL
E-mail  : radhika.k.agri@snsct.org
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Syllabus

UNIT
1
CARBOHYDRATES

Carbohydrates (Occurrence in Plants, Commercial Production, Food Uses, Healthful Properties) - mono, oligo and polysaccharides. Metabolic pathway and bioenergetics – Glycolysis and TCA cycle. Lab Experiments:  Qualitative tests for carbohydrates  Qualitative tests for proteins

UNIT
2
LIPIDS

Classification of lipids - simple, compound and derived lipids. Fatty Acids: Saturated Fatty Acids, Unsaturated Fatty Acid. Digestion and intake, Essential fatty acids, Industrial uses. Lab Experiments:  Determination of Ash content of the given food sample

UNIT
3
NUCLEIC ACID BIOCHEMISTRY & PROTEIN

DNA: Nucleoside & Nucleotide - Chargaff's rule, Watson and crick model of DNA and RNA. Amino acids and biosynthesis of protein. Lab Experiments:  Estimation of glucose (Food sample)

UNIT
4
VITAMINS AND HORMONES

Functions, sources, absorption, deficiency of Vitamins: fat-soluble vitamins and water-soluble vitamins. Information about hormones & relation between vitamins and hormones. Lab Experiments:  Determination of the calorie content of the given food sample  Isolation of starch

UNIT
5
ADVANCED TECHNIQUES FOR BIOCHEMICAL INVESTIGATION

Chromatography- TLC, GLC, HPLC and HPTLC. Basic Genetic Techniques – PCR, Blotting techniques – Southern blotting, Northern blotting, and Western blotting. Lab Experiments:  TLC

Reference Book:

REFERENCES 1. Food Biochemistry and Food Processing, Benjamin K Simpson, Wiley –Blackwell; Second edition (2012). 2. Biochemistry, LubertStryer, John L. Tymoczko, Jeremy Mark Berg, W. H.Freeman Company; Fifth edition (2002).

Text Book:

TEXT BOOKS 1. Advances in Food Biochemistry, FatihYildiz , CRC Press, New York; © 2010 by Taylor and Francis Group, LLC. 2. Fundamentals of Biochemistry, A C Deb, New central book agency (P) Ltd; Ninth Edition (2016). 3. Lehninger Principles of biochemistry, David L. Nelson, W H Freeman & Co; Sixth edition (2013).

 

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