Water, Acid – Base, Buffers, Solutions, Handerson-Hasselbalch equation. Principle of osmosis and their application.
Classification of carbohydrates - mono, di, oligo and polysaccharides. Metabolic pathways and bioenergetics – Glycolysis, TCA cycle. Classification of lipids- simple, compound and derived lipids.
DNA: Nucleoside & Nucleotide - Chargaff's rule, Watson and crick model of DNA. Structure of RNA and its type. Protein biosynthesis. Blotting techniques – Southern blotting, Northern blotting, and Western blotting.
Blood, Hemolysis, Anticoagulants, Preservation of blood, Deprotenisation of blood.
Liver function test- Serum bilirubin & Jaundice, Kidney function test, Thyroid function test and Gastric function test.
Reference Book:
1. Biochemistry, Donald Voet, Judith G. Voet, Wiley, John & Sons; third edition (2003). 2. Biochemistry, Lubert Stryer, John L. Tymoczko, Jeremy Mark Berg, W. H.Freeman Company; Fifth edition (2002). 3. Biochemistry, Christopher K. Mathews, Kensal E. van Holde, Kevin G. Ahern,Pearson Benjamin Cummings; Third edition, Book and CD-ROM edition (1999).
Text Book:
1. Biochemistry, U Satyanarayana, Elsevier Health Sciences; Fifth Edition (2017) 2. Lehninger Principles of biochemistry, David L. Nelson, W H Freeman & Co; Sixth edition (2013).
