INTRODUCTION Importance and relevance to nursing Historical perspective Concepts and terminology Principles of microbiology

GENERAL CHARACTERISTICS OF MICROBES Structure and classification of Microbes Morphological types & Size and form of bacteria Motility , Colonization & Growth andnutrition of Microbes Temperature & Moisture Blood and body fluids Laboratory methods for identification of Microorganisms Staining techniques, Gram staining, Acid fast staining, Hanging drop preparation • Culture; various medias

INFECTION CONTROL • Infection: Sources, portals of entry and exit, transmission • Asepsis • Disinfection; Types and methods • Sterilization; Types and Methods • Chemotherapy and antibiotics • Standard safety measures • Biomedical waste management • Role of Nurse • Hospital acquired infection • Hospital infection control programme Protocols, collection of samples, preparation of report and status of rate of infection in the unit hospital, nurse’s accountability, Continuing education etc.

PATHOGENIC ORGANISMS • Micro-organisms Cocci — gram positive and gram negative bacilli— gram positive and gram negative • Spirochaete • Mycoplasma • Rickettsiae • Chlamydiae • Viruses - • Fungi -Superficial and Deep mycoses • Parasites • Rodents & vectors Characteristics, Source, portal of entry, • Rodents & vectors transmission of infection, Identification of disease producing micro-organisms, Collection,handling and transportation of various specimens

IMMUNITY • Immunity-Types, classification • Antigen and antibody reaction • Hypersensitivity — skin test • Serological tests • Immunoprophylaxis • Vaccines & sera -Types & Classification, storage and handling, cold chain • Immunization for various diseases • Immunization Schedule